Quantitative real-time PCR method to detect changes in specific transcript and total RNA amounts
نویسندگان
چکیده
منابع مشابه
TaqMan real-time PCR: a reliable method to detect meat species
The aim of this study was to detect and identify meat species (beef, lamb, pork, chicken, donkey, and horse) in Kebab as a Halal meat-based food sold in on-road-restaurants of North Khorasan province (Iran) using TaqMan real-time polymerase chain reaction (PCR). Raw Kebab samples (150 samples) were obtained from on-road restaurants. The samples were prepared, DNA was extracted and TaqMan real-t...
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The vast majority of genes in humans and other organisms undergo alternative splicing, yet the biological function of splice variants is still very poorly understood in large part because of the lack of simple tools that can map the expression profiles and patterns of these variants with high sensitivity. High-throughput quantitative real-time polymerase chain reaction (qPCR) is an ideal techni...
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Accurate quantitation of the number of cells of individual bacterial species in dental plaque samples is needed for understanding the bacterial etiology of periodontitis. Real-time PCR offers a sensitive, efficient, and reliable approach to quantitation. Using the TaqMan system we were able to determine both the amount of Porphyromonas gingivalis and the total number of bacterial cells present ...
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The aim of this study was to identify different virulence pathogens using Real-Time PCR method for rapid and accurate detection of common viral diseases including Infectious Hematopoietic Necrosis (IHN), Viral Hemorrhagic Septicemia (VHS) and Infectious Pancreatic Necrosis disease (IPN) in rainbow trout broodstock have been the main generation of reproductive populations maintained in the natio...
متن کاملQuantitative Real-Time PCR
Unlike classical end-point analysis PCR, real-time PCR provides the data required for quantification of the target nucleic acid. The results can be expressed in absolute terms by reference to external quantified standards or in relative terms compared to another target sequence present within the sample. Absolute quantification requires that the efficiency of the amplification reaction is the s...
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ژورنال
عنوان ژورنال: Electronic Journal of Biotechnology
سال: 2004
ISSN: 0717-3458,0717-3458
DOI: 10.2225/vol7-issue1-fulltext-5